1. 'In a randomized, double-blinded, placebo controlled 8-week study involving 132 healthy men and women, age between 25 to 55 years, 200mg of Reishi β-glucan helped increase vigor and reduced the scores related to anxiety, depression, fatigue, and mood disturbance. Test subjects used a Profile of Mood States Short Form (POMS-SF) to report their mood state.'
The results indicated that the group receiving Reishi β-glucan is statistically different than the one receiving placebos in five POMS factors mean scores including Tension-anxiety (p<.05), Vigor-activity (p<.05), Fatigue-activity (p<.05), Depression (p<.05) and mood disturbance (p<.05)
2. 'In an open-labelled, paralleled group, randomly selected, 90 day study, involving 16 men, 11 women, (36±13 yrs) all employees of an accounting firm. 2 groups: Group 1 (given 300mg of Reishi β-glucan) and Group 2 (given 600mg of Reishi β-glucan), were asked to keep track of 9 Upper Respiratory Infection symptoms (URTI) including: headache, tiredness, weakness, fatigue, nasal congestions, sneezing, coughing, sore throat, and general aches.'
The results indicated a significant decline in URTI symptoms by both groups, both in the middle and end of study. During the interim analysis (Day 46), a decrease of 34.22% observed in group 2 receiving 600 mg per day, which was higher than 31.71% observed in group 1 receiving 300 mg per day (2.81%, p=.036). During the conclusion of the trial (Day 91), the average reduction in URTI symptoms in group 2 (receiving 600 mg/day) was 46.83%, which is significantly higher than those of in group 1 receiving 300 mg/day (42.68%) (4.15%, p=.041).
3. ‘In a randomized, double-blinded, placebo controlled 84-day study involving 126 healthy men and women, age between 18 to 55 years, were administered 200mg of Reishi β-glucan. This study’s goal was to confirm immunomodulatory properties of Reishi β-glucan. More specifically that it has both innate and adaptive immune responses due to Reishi β-glucan being a Pathogen recognition receptor agonist (PRR).’
At the conclusion of the 84-day administration period, blood samples were collected to analyze for primary outcomes, including the impact of Reishi β -glucan on various immune cells (CD3+, CD4+and CD8+ T-lymphocytes, NK cell counts/mediated cytotoxicity and serum IgA levels), as well as secondary outcomes, including various hematological biomarkers (alanine aminotransferase (ALT), aspartate aminotransferase (AST), Creatinine, red blood cell (RBC), hemoglobin (HB), hematocrit(HCT) and platelet counts) to determine the safety and tolerability of the intervention, in order to provide a comprehensive understanding of the effectiveness of Reishi β-glucan .
The key findings from this study included that Reishi β -glucan induced statistically significant modifications of the CD3+, CD4+, CD8+ T-lymphocytes and NK cells, as well as elicited a statistically significant increase in serum IgA concentration in the Reishi β-glucan group versus placebo.
'In a 12 week, randomized, double-blinded, placebo-controlled clinical study among elders 60 years and older. 200mg of Lion's Mane β-glucan improved bodily pain ( by 10.7%, p< 0.05), general health perception rating (improved by 12.9%, p<0.05), vitality rating (improved by 13.4%, p< 0.05), and emotional role functioning rating (improved by 14.4%, p< 0.05).'
1. SAITSU, Y., NISHIDE, A., KIKUSHIMA, K., SHIMIZU, K., & OHNUKI, K. (2019). Improvement of cognitive functions by oral intake of Hericium erinaceus. Biomedical Research, 40(4), 125–131. https://doi.org/10.2220/BIOMEDRES.40.125
2. Mori K, Inatomi S, Ouchi K, Azumi Y, Tuchida T. Improving effects of the mushroom Yamabushitake (Hericium erinaceus) on mild cognitive impairment: a double-blind placebo-controlled clinical trial. Phytother Res. 2009 Mar;23(3):367-72. doi: 10.1002/ptr.2634. PMID: 18844328.
3. Kürşat Kaya, Osman Çiftçi and Mustafa Namık Öztanır et al. Beta-glucan attenuates cerebral ischemia/reperfusion-induced neuronal injury in a C57BL/J6 mouse model.Braz. J. Pharm. Sci..2019. Vol. 55. DOI: 10.1590/s2175-97902019000218312
4. Alp H, Varol S, Celik MM, Altas M, Evliyaoglu O, Tokgoz O, Tanrıverdi MH, Uzar E. Protective effects of beta glucan and gliclazide on brain tissue and sciatic nerve of diabetic rats induced by streptozosin. Exp Diabetes Res. 2012;2012:230342. doi: 10.1155/2012/230342. Epub 2012 Jan 16. PMID: 22291696; PMCID: PMC3265075.
5. Baldwin KT, Carbajal KS, Segal BM, Giger RJ. Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration. Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2581-6. doi: 10.1073/pnas.1423221112. Epub 2015 Feb 9. PMID: 25675510; PMCID: PMC4345569.
6. Kuo HC, Lu CC, Shen CH, Tung SY, Hsieh MC, Lee KC, Lee LY, Chen CC, Teng CC, Huang WS, Chen TC, Lee KF. Hericium erinaceus mycelium and its isolated erinacine A protection from MPTP-induced neurotoxicity through the ER stress, triggering an apoptosis cascade. J Transl Med. 2016 Mar 18;14:78. doi: 10.1186/s12967-016-0831-y. Retraction in: J Transl Med. 2021 Feb 15;19(1):67. PMID: 26988860; PMCID: PMC4797317.
1. Chang, CJ., Lu, CC., Lin, CS.et al.Antrodia cinnamomeareduces obesity and modulates the gut microbiota in high-fat diet-fed mice.Int J Obes42, 231–243 (2018). https://doi.org/10.1038/ijo.2017.149
2. Chen, Shiu-Nan, et al. ‘Novel Application of Immunomodulatory Mushroom Polysaccharide (β-Glucan) and Triterpenes for Diabetic Wound Care’. The Eye and Foot in Diabetes, IntechOpen, 19 Nov. 2020. Crossref, doi:10.5772/intechopen.93122.
3. Liu Y, Li L, An S, Zhang Y, Feng S, Zhao L, Teng L, Wang D. Antifatigue Effects ofAntrodia cinnamomeaCultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model. Biomed Res Int. 2017;2017:9374026. doi: 10.1155/2017/9374026. Epub 2017 Mar 23. PMID: 28424791; PMCID: PMC5382311.
4. Han C, Shen H, Yang Y, Sheng Y, Wang J, Li W, Zhou X, Guo L, Zhai L, Guan Q. Antrodia camphorata polysaccharide resists 6-OHDA-induced dopaminergic neuronal damage by inhibiting ROS-NLRP3 activation. Brain Behav. 2020 Nov;10(11):e01824. doi: 10.1002/brb3.1824. Epub 2020 Sep 9. PMID: 32902155; PMCID: PMC7667329.
Vince C. Kreipke, PhD, Robert J. Moffatt, PhD, Charles J. Tanner, MA & Michael J. Ormsbee, PhD (2021) Effects of Concurrent Training and a Multi-Ingredient Performance Supplement Containing Rhodiola rosea and Cordyceps sinensis on Body Composition, Performance, and Health in Active Men, Journal of Dietary Supplements, 18:6, 597-613, DOI: 10.1080/19390211.2020.1822486
Katie R. Hirsch, Abbie E. Smith-Ryan, Erica J. Roelofs, Eric T. Trexler & Meredith G. Mock (2017) Cordyceps militaris Improves Tolerance to High-Intensity Exercise After Acute and Chronic Supplementation, Journal of Dietary Supplements, 14:1, 42-53, DOI: 10.1080/19390211.2016.1203386
Xingguo Quan, Beom Seok Kwak, Ji-Young Lee, Jin Hee Park, Anbok Lee, Tae Hyun Kim, SaeGwang Park, "Cordyceps militarisInduces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer",Evidence-Based Complementary and Alternative Medicine, vol. 2020, Article ID 9053274, 11 pages, 2020. https://doi.org/10.1155/2020/9053274
Akalesh Kumar Verma (2022) Cordycepin: a bioactive metabolite of Cordyceps militaris and polyadenylation inhibitor with therapeutic potential against COVID-19, Journal of Biomolecular Structure and Dynamics, 40:8, 3745-3752, DOI: 10.1080/07391102.2020.1850352
Sustained calm energy without forced spikes and crashes*
Human Clinical Studies:
1. ‘A randomized, triple-blind, placebo-controlled, crossover study to investigate the efficacy of a single 200mg dose of AlphaWave®L-Theanine on stress in a healthy adult population.’
16 healthy subjects (cross-over design), ranging from age 16 to 60, were administered a 200mg single dose of AlphaWave® L-Theanine and the response to an acute stress model was investigated. In the study, the single dose of AlphaWave® L-Theanine significantly increased frontal region alpha power, and significantly reduced salivary cortisol levels, compared to placebo. These changes are indicative of relaxation in the brain and demonstrate a calming response.
2. ‘The Effects of AlphaWave®L-Theanine on Relaxation, Clarity and Cognitive Function: a Human Study w/20 Subjects: a randomized, double blind, placebo controlled study. ’
For twenty subjects (10 male and 10 female), aged between 21 and 47 years, 200mg of AlphaWave® L-Theanine showed a positive effect over time on the tonic alpha power, that measures relaxed wakefulness, whereas the placebo had negative effects. The group consuming AlphaWave® also displayed reduced fatigue and significantly reduction of heart rate compared with placebo over the time period of the study. This was a further indication of the actual stress reduction effect.
Supports a positive attitude during PMS and Menopause*
1. Jackson, Philippa & Forster, Joanne & Khan, Julie & Pouchieu, Camille & Dubreuil, Séverine & Gaudout, David & Moras, Benjamin & Pourtau, Line & Joffre, Florent & Vaysse, Carole & Bertrand, Karène & Abrous, Hélène & Vauzour, David & Brossaud, Julie & Corcuff, Jean-Benoît & Capuron, Lucile & Kennedy, David. (2021). Effects of Saffron Extract Supplementation on Mood, Well-Being, and Response to a Psychosocial Stressor in Healthy Adults: A Randomized, Double-Blind, Parallel Group, Clinical Trial. Frontiers in Nutrition. 7. 606124. 10.3389/fnut.2020.606124.
2. Omidkhoda SF, Hosseinzadeh H. Saffron and its active ingredients against human disorders: A literature review on existing clinical evidence. Iran J Basic Med Sci. 2022 Aug;25(8):913-933. doi: 10.22038/IJBMS.2022.63378.13985. PMID: 36159329; PMCID: PMC9464341.
3. Pachikian, Barbara & Copine, Sylvie & Suchareau, Marlène & Deldicque, Louise. (2021). Effects of Saffron Extract on Sleep Quality: A Randomized Double-Blind Controlled Clinical Trial. Nutrients. 13. 1473. 10.3390/nu13051473.
4. Adrian L Lopresti & Stephen J Smith (2022) An examination into the mental and physical effects of a saffron extract (affron®) in recreationally-active adults: A randomized, double-blind, placebo-controlled study, Journal of the International Society of Sports Nutrition, 19:1, 219-238, DOI: 10.1080/15502783.2022.2083455